LLLI preconditioning provides a promising approach to maximize the efficacy of cardiac cell-based therapy. Although many studies have reported possible molecular mechanisms involved in LLLI preconditioning, the exact mechanisms are still not clearly understood.
OPEN FILEOur study demonstrated that PBM with the combined 630+810 nm lasers significantly stimulated cell viability, and significantly decreased PDT and apoptosis of hBM-MSCs and hASCs in vitro. We reported new in vitro evidence where PBM administered at 630 nm (one and two times, 0.6 and 1.2 J/cm2) and 630+810 nm (three times, 2.4 J/cm2) significantly increased hASC cell viability compared to its control and the PBM-treated hBM-MSC groups.
OPEN FILEHowever, the treatments combining PRP and LLLT showed significant results (P<0.05). Our results demonstrate a decrease in the time of tendon regeneration by using the two therapies combined, accelerating the healing process.
OPEN FILEIn conclusion, the present study might furnish significant suggestions for developing an efficient approach for tenocyte induction since the external administration of bFGF2 and TGF?3, along with LLLT, influences the differentiation of PB-MSCs towards the tenogenic fate.
OPEN FILETreatment with PRP combined with irradiation at 830 nm resulted in a larger number of fibroblasts and increased concentration of type I collagen, thus accelerating the healing of the injured tendon.
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